What Is the Second Line on a Pregnancy Test?

Home pregnancy tests offer a convenient way to detect pregnancy early, providing quick results in a private setting. A common question arises when a second line appears on the test, prompting users to understand its meaning. Understanding the science behind these tests helps accurately interpret the outcome.

How Pregnancy Tests Work

Home pregnancy tests detect human chorionic gonadotropin (hCG) in urine. This hormone is produced during pregnancy after a fertilized egg implants and the placenta begins to form. hCG levels start to rise rapidly after implantation, approximately 6 to 10 days post-conception, doubling every two to three days in early pregnancy.

The test strip contains antibodies that react with hCG. When urine containing hCG passes over these antibodies, a chemical reaction occurs, and a visible line appears. Most tests also include a control line, which indicates the test functioned correctly. If the control line does not appear, the test is faulty, and the result cannot be trusted.

Understanding Your Test Results

A clear second line on a home pregnancy test, alongside the control line, indicates a positive result. The color intensity of this line can vary; even a faint second line suggests a positive result, as it signifies the presence of hCG. A faint line may occur if testing very early in pregnancy when hCG levels are low, or if the urine is diluted.

Distinguishing a faint positive line from an evaporation line is important. An evaporation line is a colorless streak that can appear as urine dries, after the recommended reading time has passed. Unlike a true positive, an evaporation line lacks color and does not indicate hCG. To avoid misinterpretation, read the test results within the specified timeframe, usually three to ten minutes.

A negative result is indicated by only the control line appearing. While home pregnancy tests are accurate when used correctly, false results can occur. False positives, though rare, can happen due to certain fertility medications containing hCG, a recent pregnancy loss, or specific medical conditions. False negatives are more common and can result from testing too early, using diluted urine, or not following instructions.

What to Do Next

If a home pregnancy test shows a positive result, contact a healthcare provider for confirmation. A medical professional can confirm pregnancy through blood tests, which detect lower hCG levels earlier, or an ultrasound. This also allows for the initiation of prenatal care, including discussions about health and lifestyle adjustments.

If the test result is negative but a missed period or other pregnancy signs persist, retesting in a few days is recommended. This allows more time for hCG levels to rise if an early test was performed. If tests continue to be negative but symptoms persist or periods remain absent, consulting a healthcare provider is important to investigate other causes. Home pregnancy tests are a preliminary indicator; medical confirmation is important regardless of the result.