What Is Paternity Testing? The Science Behind It

Paternity testing determines the biological relationship between a man and a child. This scientific process uses genetic information to establish fatherhood, providing a definitive answer to questions of biological parentage.

The Science of Paternity

Paternity testing analyzes deoxyribonucleic acid (DNA), the unique genetic material of an individual. Every person inherits half their DNA from their biological mother and half from their biological father. This inheritance pattern is the fundamental principle behind paternity tests.

Scientists analyze specific DNA regions, known as genetic markers, which vary among individuals. These markers are compared between the child, the alleged father, and often the mother, to identify shared genetic sequences. If a child’s DNA markers align with those of the alleged father, it indicates a biological connection. The more genetic markers that match, the higher the probability that the man is the biological father.

Types of Paternity Tests

Paternity tests are conducted in various contexts. Legal, or “chain-of-custody,” testing adheres to strict protocols for sample collection and documentation, making results admissible in court for matters like child support or inheritance. These tests require supervised sample collection to maintain integrity.

Informational or “peace-of-mind” tests are for personal knowledge and often collected at home. While accurate for individual use, they lack the strict chain of custody needed for legal admissibility.

Prenatal paternity tests can also be performed before birth. Non-invasive prenatal paternity (NIPP) testing is most common, analyzing fetal DNA from the mother’s blood. More invasive methods, such as chorionic villus sampling (CVS) or amniocentesis, involve collecting samples directly from the placenta or amniotic fluid, but carry a small risk.

The Testing Process

Paternity test sample collection begins. The most common method involves a buccal, or cheek, swab, to collect cells containing DNA. This non-invasive method is as effective as other collection techniques, such as blood tests. Once collected, the samples are sent to a specialized laboratory for analysis.

In the laboratory, DNA is extracted from cells. Scientists then amplify specific genetic markers using Polymerase Chain Reaction (PCR), which creates millions of copies of the DNA sequences. These amplified markers are then compared between the child and the alleged father to determine if a genetic match exists. Results are typically available within a few days to a week after the samples reach the lab.

Understanding Test Results

Paternity test results are presented with a high degree of certainty. For an alleged father who is biologically related, the report will show a “probability of paternity,” typically 99.9% or higher. This indicates the statistical likelihood that the tested man is the biological father compared to a random individual.

If the alleged father is not the biological parent, the probability of paternity will be 0%. This result definitively excludes the tested man as the biological father because there are significant mismatches in the genetic markers. Modern DNA paternity tests are highly accurate due to the analysis of numerous genetic markers. These results provide reliable information regarding biological parentage.