PeproTech 315-02 is a recombinant protein widely utilized in biological research. This laboratory-produced version of Murine Macrophage Colony-Stimulating Factor (M-CSF) serves as a fundamental tool for scientists. Its standardized, pure, and consistent quality makes it a reliable reagent, allowing researchers to precisely investigate various biological processes and understand cell behavior and development.
What is Murine M-CSF
Murine M-CSF is a natural signaling protein found in the body, belonging to the cytokine family of signaling molecules. It is a homodimeric protein, consisting of two identical polypeptide subunits, each comprising 156 amino acids, with a total molecular weight of 36.4 kDa. This protein plays a fundamental role in regulating the growth, differentiation, and survival of specific cell types. It functions as a key regulator for blood monocytes, tissue macrophages, and their progenitor cells, guiding them through their developmental stages. PeproTech 315-02 is a highly pure, laboratory-synthesized version of this natural murine protein, produced in E. coli under animal-free manufacturing conditions.
How Murine M-CSF is Used in Research
PeproTech 315-02 is widely utilized across various scientific disciplines due to its specific biological activity. In cell culture studies, it is routinely employed to stimulate the proliferation and differentiation of murine M-NFS-60 cells, with an expected ED50 of ≤ 1.0 ng/ml. It is valuable for cultivating and maintaining populations of monocytes and macrophages in vitro, providing a controlled environment to study their functions.
The protein is also applied in studies investigating hematopoietic stem cell differentiation, guiding these cells to develop into specific monocyte-macrophage lineages. Researchers use it in tissue culture experiments to observe its effects on complex cellular interactions and tissue development. It also finds use in flow cytometry (FC/FACS) applications, enabling the identification and analysis of cell populations that respond to M-CSF signaling. Its role extends to exploring processes like neovascularization, as M-CSF can induce vascular endothelial growth factor (VEGF) secretion by macrophages, which mobilizes endothelial progenitor cells.
Murine M-CSF’s Role in Health and Disease
Beyond its use as a research tool, the natural form of M-CSF, including its murine variant, has broader physiological significance and connections to health and disease. M-CSF is a survival factor supporting the development and proliferation of monocytes, macrophages, and osteoclast progenitor cells. These cell types are part of the immune system and bone remodeling. Dysregulation of M-CSF levels or its signaling pathway can contribute to various conditions.
M-CSF has been implicated in modulating dermal thickness and plays roles in both male and female fertility. In the context of disease, M-CSF is observed in conditions such as infections, certain malignancies, and atherosclerosis, where its activity can influence disease progression. It also facilitates hematopoietic recovery following bone marrow transplantation, showing its role in blood cell regeneration. However, while human M-CSF can affect mouse cells, murine M-CSF does not exhibit activity on human cells.