A spore syringe contains microscopic mushroom spores suspended in a sterile liquid. These syringes are used for mycological cultivation, allowing for the introduction of spores into a suitable environment. Proper storage is important for maintaining spore quality and viability, and preventing contamination.
Ideal Storage Environment
Maintaining a cool environment is important for preserving spore viability. The optimal temperature range for storing spore syringes is generally 2°C to 8°C (35°F to 46°F). These colder temperatures slow spore metabolic activity, helping them remain dormant and extending their shelf life. Refrigeration can keep syringes viable for months.
Light exposure, especially UV light, can degrade spores and reduce viability. Store spore syringes in a dark environment or opaque containers to protect them from light. This preserves spore potency.
Managing external moisture is important. Excessive moisture can promote mold or bacteria growth on the syringe exterior or its packaging. Storing syringes in a dry environment helps prevent such issues. A clean, stable, and secure location, such as a refrigerator drawer, provides consistent conditions.
Short-Term and Long-Term Storage Techniques
For short-term storage (days to a few weeks), spore syringes can be kept in a cool, dark cupboard or a refrigerator drawer. Store them in their original packaging or a protective case to prevent physical damage and maintain sterility. Positioning the syringe horizontally helps prevent spores from settling too densely, making them easier to disperse.
For extended preservation, refrigeration is recommended for long-term storage (several months to over a year). Place syringes in airtight containers, such as zip-lock bags or sealed plastic boxes. This protects against condensation, external contaminants, and temperature fluctuations. Including a desiccant pack can absorb excess moisture.
Freezing spore syringes is not advised, as ice crystal formation can damage the spores and compromise the syringe. While some spores might survive freezing, it can lead to a significant loss of viability. Proper packaging in a cool, dark, and dry environment helps ensure the spores remain in their intended state.
Assessing Spore Syringe Viability
Before use, allow a stored spore syringe to gradually warm to room temperature. Gently shaking the syringe helps redistribute any settled spores, ensuring an even suspension. This helps in obtaining a consistent and effective sample.
Visual cues indicate if a spore syringe is compromised. A healthy syringe appears clear or translucent, with visible spores suspended or settled. Signs of contamination include unusual discoloration (green, black, or red), cloudiness, or mold growth. An unpleasant odor also signals bacterial or fungal contamination.
Under optimal storage conditions, spore syringes can remain viable for 6 to 12 months when refrigerated. Some reports indicate viability for up to 18-24 months, though germination success may decrease with age. While visual inspection provides clues, the ultimate assessment of viability comes from successful germination and growth after inoculation.