How to Properly Collect a Urine Sample

A urine sample is a bodily fluid specimen collected for medical testing, providing a non-invasive way to assess various health indicators. These samples are frequently analyzed to check for infections, monitor kidney function, detect metabolic disorders, and screen for other conditions.

Common Collection Types

Healthcare providers may request different types of urine samples depending on the specific diagnostic needs. A common method is the clean catch (midstream) sample, which aims to minimize contamination from bacteria on the skin and is often used for detecting urinary tract infections.

Other types of urine collections serve different purposes. A random sample can be collected at any time for general screening or routine health assessments. A first morning sample, collected immediately upon waking, is more concentrated and useful for tests like pregnancy detection or bacterial cultures due to higher levels of detectable substances. For comprehensive analysis of substances that fluctuate throughout the day, a 24-hour collection involves gathering all urine over a full day to assess kidney function or hormone levels.

Preparing and Collecting Your Sample

Begin by thoroughly washing your hands with soap and water to prevent introducing germs. Next, carefully open the sterile collection cup, taking care not to touch the inside or its lid. Place the lid upside down on a clean surface.

Properly cleansing the genital area before collection is an important step. For females, separate the labia and wipe from front to back using a cleansing wipe; this helps prevent bacteria from the anal area from entering the sample. For males, wipe the head of the penis with a cleansing wipe, retracting the foreskin if uncircumcised, and hold it back during collection.

To collect the midstream sample, first urinate a small amount into the toilet. This initial urine stream helps flush out any remaining contaminants from the urethra. Without stopping the flow, position the collection cup into the urine stream and collect the required amount until the cup is about half full. Remove the cup from the stream before you finish urinating and complete the remaining urination into the toilet. This technique ensures that the collected urine represents the mid-portion of the bladder’s contents, which is less likely to be contaminated.

After Collection: Storage and Delivery

Immediately after collection, securely screw the lid onto the urine container, ensuring it is tightened completely to prevent leaks. Label the specimen clearly with your full name, date of birth, and the exact date and time of collection. Accurate labeling is important for proper identification and processing at the laboratory.

If you cannot deliver the sample to the clinic or laboratory immediately, store it in a refrigerator. Refrigeration helps to slow down the growth of bacteria, which can otherwise multiply quickly and affect test results.

Most urine samples, particularly clean catch specimens, should be delivered to the lab as soon as possible, within one to two hours if not refrigerated. If refrigerated, the sample can be stored for up to 24 hours. For 24-hour urine collections, the entire collection should be kept refrigerated throughout the collection period and transported to the laboratory promptly once complete. Confirm the specific delivery instructions with your healthcare provider or the laboratory, as timely delivery helps maintain the integrity and usability of the sample.