How to Do a Clean Catch Urine Sample

A clean catch urine sample is a collection method designed to prevent external bacteria and cells from contaminating the urine specimen. This technique ensures diagnostic tests, such as urinalysis or urine cultures, yield accurate results by reflecting only what is present within the urinary tract. Following the correct steps is important to obtain a reliable sample, isolating the urine from microorganisms that naturally reside on the skin around the genitals, which could otherwise lead to misleading test outcomes.

Gathering Your Supplies

Before beginning the collection process, gather all necessary items. A sterile collection cup is typically provided by your healthcare provider or laboratory, designed to prevent contamination. Along with the cup, you will usually receive antiseptic wipes, though plain soap and water can also be used for cleaning. Have access to a clean restroom for comfortable and hygienic collection. Begin by thoroughly washing your hands with soap and water to minimize germ transfer. When opening the sterile collection cup, take care not to touch the inside of the cup or the inner surface of the lid to maintain its sterility.

Cleaning the Area

Properly cleaning the genital area significantly reduces the chance of external contamination for a clean catch sample.

For Female Anatomy

Spread the labia with one hand to expose the urethral opening. Use an antiseptic wipe, or a clean cloth with mild soap, to wipe from front to back. Use a fresh wipe for each stroke, wiping the left side, then the right, and finally the center, repeating this process two or three times. Ensure discarded wipes are placed away from the collection area to avoid re-contamination.

For Male Anatomy

If uncircumcised, gently retract the foreskin to expose the head of the penis. Use an antiseptic wipe or a clean cloth with mild soap to clean the tip of the penis, wiping in a circular motion. Discard the wipe after use. The aim is to cleanse the area around the urinary opening to remove surface bacteria that could otherwise enter the urine stream.

Collecting the Sample

The “midstream” collection technique obtains the most representative urine sample. Begin by urinating a small amount directly into the toilet; this initial flow flushes out any remaining contaminants from the urethra. Without stopping the flow, quickly place the sterile collection cup into the stream to collect the sample. Fill the cup approximately half full, typically requiring 30 to 60 milliliters of urine.

After collecting the sufficient amount, remove the cup from the stream and finish urinating into the toilet. Avoid touching the inside of the collection cup or its lid with your fingers or any part of your genital area, as this can introduce contaminants. Keeping the cup steady and maintaining a consistent urine flow into the container helps ensure the sample’s integrity.

Handling and Transporting Your Sample

Immediately after collection, securely cap the urine collection cup to prevent leakage or contamination during transport. Label the cup clearly with your full name, the date, and the exact time of collection. This labeling ensures proper identification and traceability of the specimen.

Deliver the sample to the laboratory or healthcare provider as soon as possible, ideally within one hour of collection. Prompt delivery helps maintain the sample’s integrity and prevents bacterial growth, which can alter test results. If immediate transport is not feasible, refrigerate the sample at 2-8°C (35-46°F) to slow bacterial proliferation. While refrigeration can preserve the sample, it should not exceed 24 hours, especially for cultures, as prolonged storage can still impact test accuracy.