A hemocytometer is a specialized device used to count microscopic particles, such as cells, within a liquid sample. Its primary function is to determine the concentration of cells or other tiny elements in solutions, a common task in fields like biology, medicine, and environmental science. Accurate results depend on its cleanliness. Proper cleaning maintains the hemocytometer’s integrity, extends its lifespan, and ensures reliable cell counts.
Necessary Cleaning Materials
Distilled water is used for rinsing, removing loose debris and chemical residues. A mild, laboratory-grade detergent helps dissolve and lift dried biological samples or contaminants from the glass surface.
70% or 95% isopropyl alcohol dissolves organic residues, removes water spots, and aids in rapid drying. Lint-free wipes or lens paper are essential for gentle wiping without leaving fibers. For stubborn dried materials, a soft brush or cotton swabs can dislodge debris without scratching the delicate etched grid.
Detailed Cleaning Procedures
After each use, immediately rinsing the hemocytometer and its coverslip with distilled water prevents samples from drying onto the surface. This initial rinse helps dislodge loose cells and residual liquid, making subsequent cleaning steps more effective.
Following the initial rinse, a mild detergent solution can be applied. Gently scrub the hemocytometer and coverslip with a soft brush or cotton swab, paying close attention to areas where dried material might adhere. Handle the glass components with care to avoid scratching the precision-etched counting grid, as even minor abrasions can compromise counting accuracy.
After washing with detergent, thoroughly rinse both the hemocytometer and coverslip with plenty of distilled water. This ensures all detergent traces are removed, as lingering residue can interfere with future measurements or affect cell viability.
A final rinse with 70% or 95% isopropyl alcohol is often recommended. This alcohol rinse removes water spots and organic residues, and its quick evaporation facilitates drying. For persistent stains like dried blood, a dilute bleach solution can be used, followed by extensive rinsing. Ensure proper ventilation when using stronger cleaning agents like bleach.
Drying and Storing Your Hemocytometer
After the cleaning process, proper drying of the hemocytometer and coverslip is important to prevent water spots and inhibit microbial growth. Air drying in a dust-free environment is a suitable method, allowing the surfaces to dry completely without physical contact. Alternatively, gently wiping the surfaces with lint-free wipes or lens paper can expedite the drying process. Avoid using standard paper towels, which can leave behind fibers or scratch the delicate glass surface.
Once completely dry, the hemocytometer and its coverslip should be stored in a protective manner. Keeping them in a designated protective case, a clean drawer, or a dust-free container helps to prevent scratches, contamination, and accidental breakage. Storing these components separately from other lab equipment can further reduce the risk of damage, ensuring the hemocytometer remains in optimal condition for consistent and accurate cell counting.