Do Rats Have Balls? The Unique Anatomy of Rat Testicles

Male rats, often called bucks, possess testicles housed within a scrotal sac. These organs are responsible for the dual functions of sperm production and hormone secretion. Understanding the specific characteristics of rat testicles provides insight into this rodent’s unique reproductive biology.

The Unique Anatomy of Rat Testicles

The testicles of a male rat are disproportionately large compared to its overall body mass. They can constitute approximately 4.4% of its total body weight, a ratio among the largest observed in mammals. This striking size difference reflects evolutionary pressure and intense competition between males to fertilize females. A larger testicular volume allows for greater sperm production, granting a competitive edge in their polygamous mating system.

A distinguishing anatomical feature is the rat’s inguinal canal, the passageway connecting the abdominal cavity to the scrotum, which remains open throughout the animal’s life. This open canal allows the testicles to freely move, or retract, into the abdomen for protection and thermoregulation. Unlike most other mammals where this canal closes, the rat’s anatomy permits constant movement. Consequently, a male rat’s scrotum may occasionally appear empty, depending on whether the testes are resting externally or retracted internally.

Reproductive Function and Hormones

The primary biological roles of the testicles are to produce male gametes and to synthesize androgen hormones. Within the testes, spermatogenesis occurs in the seminiferous tubules, where sperm cells are continuously generated. The production of viable sperm requires a high local concentration of the hormone testosterone.

Testosterone, the principal androgen hormone produced by specialized Leydig cells within the testes, is necessary to maintain the entire process of sperm development and maturation. High levels of this hormone are required for germ cells to progress through meiosis and for the final release of mature sperm. Beyond sperm production, testosterone influences the development of male secondary sexual characteristics and plays a role in various behaviors, including territorial marking and aggression.

Distinguishing Male and Female Rats

Identifying the sex of a rat is most reliably done by examining the anogenital distance (AGD), the space between the genital opening and the anus. Male rats have an AGD that is significantly longer than that of female rats, often measuring about twice the distance. This difference is already visible in newborns.

As the rats mature, additional features become apparent to help confirm the sex. Male rats develop a visible scrotum as the testes fully descend, typically between 30 and 40 days of age. Conversely, female rats possess a second opening, the vaginal opening, located just below the urethral opening, and they are the only sex to develop six pairs of visible nipples along their underside. These clear anatomical differences make it straightforward to distinguish between a male buck and a female doe.