Can an Expired Pregnancy Test Give a False Positive?

The home pregnancy test (HPT) is a common diagnostic tool that identifies the presence of human chorionic gonadotropin (hCG) in urine. This hormone is produced by the placenta shortly after a fertilized egg implants, making it a reliable marker of pregnancy. While an expired test is almost always less reliable, the specific concern is whether it can generate a false positive result, indicating a pregnancy that does not exist.

How Home Pregnancy Tests Detect Pregnancy

Home pregnancy tests operate using a lateral flow immunoassay. The test strip contains specialized antibodies, which are proteins designed to recognize and bind exclusively to the hCG molecule. When urine is applied to the test, it travels up the absorbent material, carrying mobile antibodies tagged with a colored particle.

These tagged antibodies encounter the hCG molecules in the urine, forming a complex that moves along the strip. The complex then reaches a designated test zone, where stationary antibodies are anchored. This stationary group captures the hCG-antibody complex, concentrating the colored particles and forming the visible positive line. A separate control line appears whether hCG is present or not, confirming the test absorbed enough urine and that the chemical reagents are active.

What Happens When a Test Expires

The accuracy of a home pregnancy test relies entirely on the integrity and responsiveness of the antibodies embedded in the strip. Antibodies are proteins susceptible to degradation over time, especially when exposed to heat or excessive moisture. The expiration date on the packaging is the manufacturer’s guarantee that these proteins will function as intended.

Once a test passes its expiration date, the primary risk is that the antibodies lose their ability to bind effectively to hCG. This loss of function makes the test significantly less sensitive, often resulting in a false negative result. Severe degradation can also cause the control line to fail, indicating an invalid result.

Expiration is generally an unlikely cause of a true false positive result. However, when reagents break down, there is a remote possibility of “non-specific binding.” Degraded antibodies may stick to the test line even without hCG present. This chemical malfunction can, in rare instances, mimic a positive result, but the overwhelming consequence of expiration is loss of sensitivity, leading to missed positive results rather than incorrect positive ones.

Sources of False Positive Results

Since expiration is not the most common reason for an incorrect positive result, other factors must be considered when a positive test is unexpected. One frequent cause is a chemical pregnancy, which is a very early miscarriage occurring shortly after implantation. The fertilized egg produces enough hCG to trigger a positive result before the pregnancy is lost, meaning the test accurately detected the hormone.

Certain medications can also lead to a false positive, most notably fertility drugs that contain synthetic hCG, such as those used to trigger ovulation. Since the test cannot distinguish between the synthetic hormone and the naturally produced one, the result will be positive. Evaporation lines are another common source of confusion, appearing when the test is read after the suggested time window. Residual moisture leaves a faint, colorless line often mistaken for a true positive.

In rare instances, certain medical conditions can cause the body to produce hCG, even in the absence of pregnancy. These conditions include specific types of ovarian cysts, pituitary problems, or tumors originating from reproductive tissues. Women who are perimenopausal or postmenopausal may also have naturally elevated, low levels of hCG that could register as a faint positive on a sensitive test.

Interpreting Test Results and Next Steps

If a positive result appears on an expired test, or if the result is questionable, the next step is to retest immediately with a new, non-expired product. The new test will verify the initial finding and eliminate doubt regarding the integrity of the device.

If the positive result is confirmed on a fresh test, or if there is persistent doubt, contacting a healthcare provider for professional confirmation is necessary. A provider can order a quantitative blood test, which measures the exact concentration of hCG, offering a definitive answer. They can also use ultrasound imaging to confirm the location and viability of a pregnancy.