A Nunclon Delta Surface 6 Well Plate is a specialized laboratory tool used in scientific research for growing cells. These plates provide an optimized environment for various cell types to adhere, grow, and proliferate. Manufactured from high-quality polystyrene, they are a consumable for biological experiments requiring controlled cell culture conditions.
Understanding Nunclon Delta Surface Technology
The “Nunclon Delta Surface” refers to a specific surface treatment applied to polystyrene cell culture plates. This proprietary modification makes the normally hydrophobic polystyrene surface more hydrophilic, enhancing cell attachment and spreading. The surface is created through a plasma treatment process, resulting in a negatively charged surface that mimics the properties of the extracellular matrix.
This unique surface optimizes the interaction between cells and the plate, making it suitable for a broad spectrum of adherent cell types. The enhanced hydrophilicity and negative charge promote robust cell adhesion, which supports consistent cell growth and proliferation in laboratory settings. Every lot of Nunclon Delta surface products undergoes rigorous validation to confirm consistent cell growth across multiple cell lines, ensuring reliable and reproducible experimental outcomes.
The Structure and Purpose of 6 Well Plates
A 6 well plate is a flat, rectangular plate containing six individual wells, each serving as a separate reaction vessel. Each well typically has a round shape with a flat bottom, providing a consistent surface area for cell attachment and growth. The culture area per well is approximately 9.5 to 9.6 cm², with a suggested working volume of around 3 mL per well for cell culture media.
This format is widely used in laboratories, offering a balance between sufficient surface area for cell growth and the ability to run multiple experimental conditions or replicates simultaneously. The larger individual well size, compared to plates with more wells, is beneficial for growing adherent cell lines that require ample space. The design often includes features like raised well rims to minimize cross-contamination and alphanumeric codes for easy sample identification.
Common Applications in Biological Research
Nunclon Delta Surface 6 Well Plates are frequently used across various biological research domains due to their optimized surface and convenient format. Common applications include:
- Cell biology, cancer research, and stem cell studies for basic cell growth and proliferation assays.
- Drug discovery, including drug screening and toxicity testing, where consistent cell behavior is important for accurate results.
- Gene expression studies, allowing analysis of how genes are turned on or off under different conditions.
- Imaging-based experiments, as the larger well size and wide flat bottom provide a clear view for microscopic analysis.
- Producing small batches of proteins or RNA, making them a versatile tool for molecular biology applications.