C6 cells are a widely utilized cell line in scientific research. These cells provide a consistent and reproducible system for investigating complex biological processes in a controlled laboratory environment. Their widespread adoption underscores their adaptability and relevance in advancing our understanding of cellular functions and disease mechanisms.
Origin and Key Characteristics
C6 cells originated from a brain tumor induced by N-nitrosomethylurea in an adult male Wistar-Furth rat, making them a rat glioma cell line that mimics certain aspects of glial cells found in the brain. They exhibit a fibroblast-like morphology and adhere to culture surfaces, growing in a monolayer.
These cells display a doubling time of approximately 25 to 30 hours. Cytogenetic analysis indicates a diploid male karyotype, suggesting a degree of genetic stability. C6 cells also overexpress certain glioma-associated markers, such as S-100 protein and glial fibrillary acidic protein (GFAP).
Broad Research Applications
C6 cells are broadly employed in neuroscience studies, particularly for understanding glial cell function and dysfunction. Their glial-like characteristics make them suitable for investigating cellular responses within the central nervous system. This includes studies on neurotoxicity, where researchers assess the effects of various substances on brain cells.
The cell line is also extensively used in cancer research, especially for studying brain tumors like glioma. C6 cells can simulate the high growth rate, vascularization, and infiltrative nature observed in human glioblastoma multiforme. This allows for the development and testing of new therapeutic agents and strategies against brain cancers. C6 cells also serve as a model in toxicology studies to evaluate the harmful effects of chemicals and compounds, contributing to safety assessments and understanding mechanisms of toxicity.
Specific Research Areas and Models
C6 cells are also used in more specific research areas. They are used to study neuroinflammation, a process involving the activation of brain-resident cells like microglia and astrocytes. Researchers can use C6 cells to investigate how inflammatory stimuli impact brain health and contribute to neurological disorders.
The cell line is also relevant for examining blood-brain barrier integrity. While C6 cells themselves are not endothelial cells that form the barrier, they can be used in co-culture models with other cell types to simulate the complex interactions at this barrier. This helps in understanding how various conditions or compounds might affect the barrier’s permeability. Additionally, C6 cells are employed in drug delivery mechanism studies, particularly for therapies targeting the brain. Researchers can assess how different drug formulations, including nanoparticles, interact with glial cells and potentially cross barriers to reach their intended targets within the central nervous system.